Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker

Table 1

Primer sequences used in quantitative real-time reverse transcription polymerase chain reactions.

Gene target	Sequence
Bmi-1	Forward: 5-TGGACTGACAAATGCTGGAGA-3
	Reverse: 5-GAAGATTGGTGGTTACCGCTG-3
Nanog	Forward: 5-AAGGCCTCAGCACCTACCTA-3
	Reverse: 5-TGCACCAGGTCTGAGTGTTC-3
Oct4	Forward: 5-GGCCACACGTAGGTTCTTGA-3
	Reverse: 5-GGCTGAATACCTTCCCAAATAGA-3
ALP	Forward: 5-TTCAAACCGAGATACAAGCACT-3
	Reverse: 5-GGGCCAGACCAAAGATAGAG-3
RUNX2	Forward: 5-TGGTTACTGTCATGGCGGGTA-3
	Reverse: 5-TCTCAGATCGTTGAACCTTGCTA-3
LPL	Forward: 5-TCATTCCCGGAGTAGCAGAGT-3
	Reverse: 5-GGCCACAAGTTTTGGCACC-3
PPAR-γ	Forward: 5-ATGGTGGACACGGAAAGCC-3
	Reverse: 5-CGATGGATTGCGAAATCTCTTGG-3
GAPDH	Forward: 5-AGGTCGGAGTCAACGGATTTG-3
	Reverse: 5-AGGCTGTTGTCATACTTCTCAT-3