miRNAs in Extracellular Vesicles from iPS-Derived Cardiac Progenitor Cells Effectively Reduce Fibrosis and Promote Angiogenesis in Infarcted Heart

Figure 6

miR-373 mimic improved cardiac function and angiogenesis and attenuated cardiac fibrosis after MI. (a) Representative M-mode echocardiography images from miRNA mimic negative control- (NC-) treated mice and miR-373 mimic-treated mice 30 days post-MI. FS (b) and EF (c) are shown; , in the NC group and in the miR-373 mimic group. (d) Representative Masson’s trichrome-stained sections of hearts from NC-treated mice and miR-373 mimic mice. (e) Quantitative analysis of fibrosis post-MI. (f) Vessel density was assessed by α-SMA-positive staining (green) of vascular structures. . (g) Quantitative estimate of arteriole density. . in each group. Schematic depiction of mechanisms of protection by EV-CPC^ISX-9: role of miR-373 in suppressing fibrosis by targeting two genes, GDF-11 and ROCK-2, and inhibiting myofibroblast differentiation. Myocyte proliferation and angiogenesis were also promoted by EV-CPC^ISX-9.

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