Stem Cells International / 2019 / Article / Tab 3 / Review Article
Meta-Analysis and Evidence Base for the Efficacy of Autologous Bone Marrow Mesenchymal Stem Cells in Knee Cartilage Repair: Methodological Guidelines and Quality Assessment No. of studies References Studies used cultured BM-MSCs Type of the culture media DMEM supplemented with 10% FBS 6 [25 , 28 , 44 , 50 –52 ] DMEM supplemented with 15% FCS 2 [24 , 27 ] α -MEM supplemented 10% HyClone bovine serum2 [46 , 48 ] (DMEM) supplemented with 15% patient’s serum 1 [41 ] α -MEM+15% patient’s serum2 [55 , 56 ] (DMEM)+10% PL 1 [23 ] α -MEM+5% platelet lysate+1 ng/ml bFGF1 [29 ] Studies used noncultured BM-MSCs Centrifugation method BMAC Harvest Smart PreP2 System 5 [31 –34 , 39 ] MarrowStim Concentration Kit 5 [26 , 30 , 36 , 37 , 62 ] Magellan Autologous System (Arteriocyte) 2 [35 ] 1000 g for 30 minutes 1 [25 ] 1000 g for 10 minutes 1 [23 ] 1500 g for 20 minutes 2 [46 , 48 ] 3000 rpm for 3 minutes 1 [52 ] 3200 rpm for 15 minutes 1 [42 ] 3500 rpm for 6 minutes 1