Meta-Analysis and Evidence Base for the Efficacy of Autologous Bone Marrow Mesenchymal Stem Cells in Knee Cartilage Repair: Methodological Guidelines and Quality Assessment

Table 3

	No. of studies	References
Studies used cultured BM-MSCs
Type of the culture media
DMEM supplemented with 10% FBS	6	[25, 28, 44, 50–52]
DMEM supplemented with 15% FCS	2	[24, 27]
α-MEM supplemented 10% HyClone bovine serum	2	[46, 48]
(DMEM) supplemented with 15% patient’s serum	1	[41]
α-MEM+15% patient’s serum	2	[55, 56]
(DMEM)+10% PL	1	[23]
α-MEM+5% platelet lysate+1 ng/ml bFGF	1	[29]
Studies used noncultured BM-MSCs
Centrifugation method
BMAC Harvest Smart PreP2 System	5	[31–34, 39]
MarrowStim Concentration Kit	5	[26, 30, 36, 37, 62]
Magellan Autologous System (Arteriocyte)	2	[35]
1000 g for 30 minutes	1	[25]
1000 g for 10 minutes	1	[23]
1500 g for 20 minutes	2	[46, 48]
3000 rpm for 3 minutes	1	[52]
3200 rpm for 15 minutes	1	[42]
3500 rpm for 6 minutes	1