Acute Leukemia Induces Senescence and Impaired Osteogenic Differentiation in Mesenchymal Stem Cells Endowing Leukemic Cells with Functional Advantages

Figure 1

Leukemic cells increase senescence-associated β-galactosidase (SA-β-Gal) activity in MSC. (a) SA-β-Gal activity in MSC cocultured with REH cells (LN) or REH-CM (REH-CM LN) was measured. As controls, we have used MSC in 10% FBS (for the LN) and 1% FBS (for the REH-CM LN). Inset: magnification of the LN, showing absence of SA-β-Gal activity in REH cells. (b) Percentage of positive cells for SA-β-Gal activity in the different culture conditions ( values: Student -test-Mann-Whitney test; ). (c) qRT-PCR quantification of p53 and p16 gene expression in MSC cultured in the LN and REH-CM LN for three days. Results represent three independent experiments done in duplicates. Results are expressed as ( values: nonparametric one-way ANOVA; ns: nonsignificant, ).

(a)

(b)

(c)