The Antibiotic Bacitracin Protects Human Intestinal Epithelial Cells and Stem Cell-Derived Intestinal Organoids from Clostridium difficile Toxin TcdB

Figure 3

Bac preserves epithelial integrity of CaCo-2 monolayers from TcdB activity. CaCo-2 cells grown as epithelial monolayers on filters in a Transwell chamber were preincubated with or without Bac (2 mM) for 30 min at 37°C. Then, TcdB (6 ng/ml) was added apically. For control, cells were left untreated. The epithelial integrity was recorded by measuring the transepithelial electrical resistance (TEER) every 35 min over a time course of 20 h.