Strontium Promotes the Proliferation and Osteogenic Differentiation of Human Placental Decidual Basalis- and Bone Marrow-Derived MSCs in a Dose-Dependent Manner

Figure 3

Cytotoxic effects of strontium on human BM-MSCs. (a) Live/dead staining of human BM-MSCs cultured with different concentrations of strontium; green fluorescence: live cells; red fluorescence: dead cells; scale bar: 500 μm. (b) Cell apoptosis analysis of human BM-MSCs. The percentages of the following cell populations are indicated on the respective density plots: necrotic cells (Annexin V-negative and PI-positive) are located in the upper left (C1), late apoptotic cells (Annexin V-positive and PI-positive) in the upper right (C2), viable cells (Annexin V-negative and PI-negative) in the lower left (C3), and early apoptotic cells (Annexin V-positive and PI-negative) in the lower right (C4) quadrants, respectively. GM: growth medium. All of these results are representative of three independent experiments.

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