Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin
Evaluation of AC-GlcNAc coatings. (a) Structure of AC-GlcNAc. (b) Evaluation of AC-GlcNAc coating on polystyrene dishes using the interaction between biotinylated sWGA and AC-GlcNAc. The amount of biotinylated sWGA on dishes was measured by HRP-neutravidin and TMB solution (absorbance at 450 nm). (c) Western blots demonstrating expression of cell-surface vimentin in human immortalized MSCs. (d) UE7T-13 adherence to AC-GlcNAc-coated dishes at 37 (left) and 4°C (right). ,.