Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin
The interaction between cell-surface vimentin and AC-GlcNAc is responsible for the adhesion of UE7T-13 cells to coated dishes. (a) Inhibition of adhesion by AC-GlcNAc at 37 (left) and 4°C (right), . (b) Expression of vimentin in knockdown and control cells. (c) Inhibition of adhesion by vimentin knockdown. Adherence of vimentin-knockdown and control cells to AC-GlcNAc 5-coated dishes at 4°C, ..