The Different Effects of IFN-β and IFN-γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

Figure 4

IFN-β interacted with IFN-γ to promote TRAIL expression and counteracted IDO1 expression initiated by IFN-γ in AFMSCs by regulating the phosphorylation of STAT1/2. 1-MT downregulated TRAIL expression in IFN-β- and IFN-γ-primed AFMSCs by inhibiting the phosphorylation of STAT1/2, but upregulated the IDO1 expression in IFN-γ-primed AFMSCs by intensifying the phosphorylation of JNK/MAPK38 (Supplemental Figure S3). (a) AFMSCs were primed for 12 hours with the indicated combinations of cytokines (20 ng/ml each) with or without Jak1 inhibitor (15 μM). The protein levels of TRAIL, IDO1, and phosphorylated STAT1/2 were determined by western blotting analysis. (b) AFMSCs were treated as described in (a), in addition to setting a 1-MT (0.5 mM) combination. Results were representative of three independent experiments.

(a)

(b)