Stem Cells International / 2019 / Article / Fig 5

Research Article

The Different Effects of IFN-β and IFN-γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

Figure 5

IFN-primed AFMSCs inhibited the proliferation of H460 cells ex vivo. (a) H460 cells () were cocultured for 36 hours with AFMSCs or IFN-primed AFMSCs at different target-to-effector cell ratios. The effects of IFN-primed AFMSCs on LDH releases were determined. (b) AFMSCs were exposed to the indicated combinations of cytokines (20 ng/ml each) with or without Jak1 inhibitor (15 μM)/1-MT (0.5 mM) for 12 hours, then cocultured with double the number of H460 cells in the presence or absence of anti-TRAIL antibody (20 μg/ml) for 36 hours. The cell viability of each group was determined by CCK-8 counting. (c, d) The coculture experiment was prepared as described in (b) using H460 hrGFP cells instead of H460 cells. The representative photographs under a fluorescence microscope are shown (c), and the viability of H460 cells was further confirmed using Cell Imaging Multi-Mode Reader (d). Scale bars 100 μm. The results in (b, d) were shown as a percentage of the value against AFMSC control. The results were representative of three independent experiments (, , and , ).
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