The Different Effects of IFN-β and IFN-γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

Figure 7

Apoptotic H460 cells initiated the expression of TRAIL in AFMSCs, which might provide a sustained antitumor effect. (a) The expression trait of TRAIL in IFN-primed AFMSCs was investigated by western blotting. IFN-β+IFN-γ-primed AFMSCs showed reduced expression of TRAIL after withdrawal of IFNs but continuously expressed TRAIL when primed again with IFNs. (b) AFMSCs were primed for 12 hours by apoptotic H460 cells, its culture media, or apoptotic H460 cells pretreated with DNase or RNase. The expression of TRAIL in AFMSCs was analyzed by western blotting. Results were representative of three independent experiments. (c) Graphic model describing the antitumor mechanism of IFN-primed AFMSCs and the effect of 1-MT on the antitumor activity of IFN-primed AFMSCs.

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