Titanium Surface Properties Influence the Biological Activity and FasL Expression of Craniofacial Stromal Cells

Figure 1

Isolation, characterization, and differentiation of MSCs. (a) Schematic representation of MSCs isolated from the mandibular bone and expanded in vitro. (b) Immunophenotypical characterization performed through FACS analysis on MSCs against mesenchymal surface antigens. (c) Alizarin red staining carried out on undifferentiated and osteogenic differentiated MSCs. (d) Alcian blue and fast red staining on MSCs induced towards chondrogenic differentiation. On the right, immunofluorescence analysis shows positive staining for collagen II in chondrogenic differentiated MSCs. (e) The presence of lipid-rich vacuoles was evaluated through Oil Red O staining in undifferentiated and differentiated MSCs.

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