The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways

Figure 3

The effects of CTP-CM on MAPK signaling pathways of hDPSCs. All cells were cultured for 60 min, and CTP-CM was added at 60, 30, 15, and 0 min, namely, 0, 15, 30, and 60 min groups. The protein levels of ERK, p-ERK, P38, p-P38, JNK, and p-JNK were determined by western blot (a). Quantitative analysis of protein bands was done by ImageJ. The levels of p-ERK/ERK, p-JNK/JNK, and p-P38/P38 were normalized by GAPDH (b). The hDPSCs were pretreated with MAPK signal inhibitors for 1 hour and then were treated with CTP-CM for the indicated times, when cells cultured in complete medium were treated as the control group. The levels of p-ERK/ERK, p-JNK/JNK, and p-P38/P38 were investigated by western blot (c). Quantitative analysis of p-ERK/ERK, p-JNK/JNK, and p-P38/P38 was performed (d). The protein levels of P-JNK, P-P38, and P-ERK were predicted by immunofluorescence staining (e–g). and .

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