Kupffer Cells Promote the Differentiation of Adult Liver Hematopoietic Stem and Progenitor Cells into Lymphocytes via ICAM-1 and LFA-1 Interaction
Detection of LSK cells in the different developmental stages of the liver and bone marrow. (a) The proportion of c-kit+ sca-1+ cells in the flow plot is gated from the fetal liver and newborn liver Lin- cells (). (b) Statistical analysis for the percentage of LSK cells gated from the Lin- cells in the different developmental stages of the liver. (c) Flow cytometric plots show the percentage of LSKs among the Lin- cells from the bone marrow or liver of young and adult mice (). (d) Statistical analysis for the percentage of LSK cells gated from the Lin- cells in the different developmental stages of the liver and BM. (e, g) Hematopoietic colony formation of the mononuclear cells (e) or LSK cells (g) from the adult liver or bone marrow (the picture shows a single colony in a well of 24-well cell culture plate). A total of bone morrow or liver mononuclear cells was freshly isolated from adult C57BL/6j mice, and bone marrow or liver LSK cells were sorted by FACS and plated into complete methylcellulose medium and incubated for 10 to 14 days. The number of colonies (≧50 cells are defined as one clone) was counted under an inverted phase contrast microscope. GM-CFU: the added cytokines included SCF, IL-3, FLT-3-L, IL-7, and GM-CSF. M-CFU: the added cytokines included SCF, IL-3, FLT-3-L, IL-7, and M-CSF. Picture original magnification: ×20 (e, g). Bar: 200 μm. (f, h) Statistical analysis for the number of GM-CFU and M-CFU from MNCs or LSK cells of BM and liver (). All colonies were counted in a well of 24-well cell culture plate. Bars represent the of three independent experiments. ;.
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