Kupffer Cells Promote the Differentiation of Adult Liver Hematopoietic Stem and Progenitor Cells into Lymphocytes via ICAM-1 and LFA-1 Interaction

Figure 3

Kupffer cells promote liver extramedullary hematopoiesis. (a, b) Flow cytometric plots show the proportion of LSKs in Lin^- cells and LT-HSCs (LSK Flk2^- CD34^-) in LSKs from the BM or liver of PBS or LPS-treated mice (). Mice were intraperitoneally injected with LPS (10 μg/mL) or PBS for three days. (c, d) The statistical percentage of LSKs in Lin^- cells and LT-HSCs in LSK cells from the liver or BM of mice treated with PBS or LPS (left). Statistical analysis of the absolute number of liver or BM LSK and LT-HSC cells (right). (e) Flow cytometric plots show the proportion of LSKs in Lin^- cells in the liver, BM, and spleen from the PBS or CL-treated groups. (f) Proportion of liver LSKs in the control group, LPS-treated group, and kupffer cell-depleted LPS-induced liver extramedullary hematopoiesis group (CL+LPS). (g) The statistical picture of the ratio of liver LSK cells to Lin^- or mononuclear cells from the PBS, LPS, and LPS+CL groups. The data represent three independent experiments with 3-5 mice per group. Bars represent . ; ; .

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