Rat Hippocampal Neural Stem Cell Modulation Using PDGF, VEGF, PDGF/VEGF, and BDNF

Figure 3

Nestin expression after 14-day treatment. (a) DMEM undifferentiated control cells. (b) B27-positive differentiating control. (c) HNSC cultured with base media and 20 ng/mL of VEGF/PDGF. (d) HNSC cultured with base media and 100 ng/mL of VEGF/PDGF. (e) Negative staining control. Primary antibody omitted. (f) HNSC positive staining control. All cells stained with a primary nestin antibody and an AF568 secondary antibody (red). Nuclei stained with a Hoechst antibody (blue). (g) Nestin-positive cell percentage. Graph showing the percentage of nestin-positive cells for each growth factor treatment group at 20 ng/mL and 100 ng/mL vs. positive and negative controls after 14 days of treatment. ; ; ; and ; significantly different from DMEM (); ^xsignificantly different from all treatments except for DMEM ().

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