Rat Hippocampal Neural Stem Cell Modulation Using PDGF, VEGF, PDGF/VEGF, and BDNF

Figure 5

(a–f) NF200 expression after 14-day treatment. PDGF/VEGF at 100 ng/mL has expression levels of NF200 similar to those of B27. (a) DMEM undifferentiated control cells. (b) B27-positive differentiating control. (c) HNSC cultured with base media and 20 ng/mL of VEGF/PDGF. (d) HNSC cultured with base media and 100 ng/mL of VEGF/PDGF. (e) Negative staining control. Primary antibody omitted. (f) SHSY-5Y positive staining control. All cells stained with a primary GFAP antibody and an AF488 secondary antibody (green). Nuclei stained with a Hoechst antibody (blue). (g) NF200-positive cell percentage. Graph showing the percentage of NF200-positive cells for each growth factor treatment group at 20 ng/mL and 100 ng/mL vs. positive and negative controls after 14 days of treatment. ; ; ; and ; significantly different from DMEM ( value < 0.001); ^{^}significantly different from all treatments except for VEGF/PDGF (100 ng/mL) (); ^xsignificantly different from all treatments except for B27.

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