Dual Inhibition of BMP and WNT Signals Promotes Pancreatic Differentiation from Human Pluripotent Stem Cells

Figure 2

BMPi regulated pancreatic differentiation via decreased WNT activity. (a) The expression levels of WNT downstream genes (including WNT3, TWIST1, AXIN2, MYC) were checked between the control group and BMPi group (LDN193189) in PP stage. represented for value < 0.01 and for value < 0.001 by Student’s -test (). (b) Protein levels of PDX1, active beta-catenin, and total beta-catenin were analyzed by Western blotting in the control group and the BMP inhibitor group in PP (). (c, d) The protein of total beta-catenin and active beta-catenin were costained with PDX1 to show the intracellular localization.

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