Dual Inhibition of BMP and WNT Signals Promotes Pancreatic Differentiation from Human Pluripotent Stem Cells

Figure 3

Inhibition of WNT could facilitate the generation of pancreatic progenitor cells. (a) Immunofluorescence results exhibited the control group (no BMP inhibitor) and WNTi (using WNT inhibitor XAV939) at PP stage with pancreatic marker PDX1, liver marker AFP, and intestine marker CDX2. DAPI (blue) represented all cells for internal control. Scale bars for enlargement inset, 50 μm. (b) Costaining PDX1 and NKX6-1 represented bona fide pancreatic progenitors in WNTi (using WNT inhibitor IWR-1) condition. (c) Flow cytometry showed the analysis of PDX1-postive cells.

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