Morphological and Molecular Analysis of In Vitro Tubular Structures from Bovine Yolk Sac-Derived MSCs

Figure 3

Photomicrographs of tubular structures formed from bYS-MSCs; electronic microscopy and transmission electronic microscopy. (a) Tubular structures formed on the plate; (b) tubular structure; (c) picrosirius staining, note bundles of strongly acidophilus collagen fibers; (d) HE staining of well-defined lumen; (e) transmission electronic microscopy analysis, cells grouped with broad and granular cytoplasm, the presence of cytoplasmic organelles and a large core; (f) and (g) microscopy analysis, note the irregular surface with uneven elevation, regions with spongy and/or scaly appearance, surrounded by a membrane of apparent connective tissue and tissue similar to blood vessels.

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