MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells

Figure 4

Analyses of erythropoiesis. Single-cell suspensions from 6-, 10-, and 14-day-old EBs were seeded into erythroid CFU selective media for 14 days. The frequency of CFU-E/BFU-E in wild-type and mutant p38α EBs is shown (a). We also determined the hemoglobin level based on their pseudoperoxidase activity (b) and the frequency of EBs with visible erythroid clusters in wild-type and mutant p38alpha cells (c). A representative EB with red-coloured clusters of erythroid cells is also shown (d). Data are presented as from a minimum of four independent experiments. Statistical significance was determined by ANOVA with post hoc Bonferroni’s multiple comparison test; asterisk “” indicates a statistical significance of .

(a)

(b)

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