MAPK p38alpha Kinase Influences Haematopoiesis in Embryonic Stem Cells

Table 1

Primers for the particular markers of haematopoiesis used in the study.

Marker	Sequence	Length	Annealing temperature	Used as	Reference
GAPDH	AAGGGCTCATGACCACAGTC	252	62	Reference gene
	CATACTTGGCAGGTTTCTCCA
CD34	TGGGTAGCTCTCTGCCTGAT	193	58	Hematopoietic progenitors	[17]
	TGGCTGGTGTGGTCTTACTG
CD38	CTGTGGTGTGGTCCAAGTGA	248	54,6	Hematopoietic progenitors	[18]
	TGGCAGGCCTGTAGTTATCC
CD150	GAGAACGTTTCTGTTCAGCAAT	138	61	Marker of hematopoietic progenitors/HSC	[19]
	CTTCACTGTGCAGGCCAACAGC
C/EBPα	CGACTTCTACGAGGTGGAGC	89	61,7	Marker of myelopoiesis	[20]
	GAAAGCCAAAGGCGGCGTTG
c-Kit	AGTGTGTGGCAGAGGGATTC	274	55,7	Marker HSC	[14]
	GCCTGGATTTGCTCTTTGTTGTTA
Epo	CCTCATCTGCGACAGTCGAG	79	62	Erythropoietin	[21]
	ACAACCCATCGTGACATTTTCT
EpoR	GCCCCCTCTGTCTCCTACTT	364	62	Receptor for erythropoietin	[14]
	CGGTGATAGCGAGGAGAACC
Eklf/Klf1	GGACTTCCTCAAGTGGTGGC	135	61,8	Marker of the switch from embryonic to adult haematopoiesis	[22]
	GTCACGTCCCTCTCATCGTC
Etv2	AACTAACCACCGAGGTCCCA	170	62,5	TF connected with differentiation of hemangioblast into hematopoietic lineage	[23]
	TCATTCCCGGCTTCCTCTTG
Flk1 (KDR)	CTACAGACCCGGCCAAACAA	152	54,5	Marker of hemangioblast	[24]
	CAGCTTGGATGACCAGCGTA
GATA1	GAAGCGAATGATTGTCAGCA	427	61,5	Marker of erythroid cells	[25, 26]
	CAGCAGAGGTCCAGGAAAAG
GATA2	GGGAGTGTGTCAACTGTGGT	276	61	Marker of hematopoietic progenitors	[27]
	GCCTGTTAACATTGTGCAGC
G-CSF-R (v1)	TGGCCCTGATGTAGTCTCTCA	149	59,9	Receptor for granulocyte colony-stimulating factor; marker of granulocyte development	[28]
	AAGGCAGGTGGAACACCAGA
G-CSF-R (v2)	CCGACTGTCAGTACCAAGGG	196	59,9	Receptor for granulocyte colony-stimulating factor; marker of granulocyte development	[28]
	AGTCTGATGGTGGGGGCAAC
Hbb γ	TTGTCCTCTGCTTCTGCCAT	188	57	Embryonic hemoglobin	[26, 29]
	AGCACATTACCCAAGAGTTTG
Hbb ζ	AGAAGGCAGCTATCACAAGCATC	301	59,5	Embryonic hemoglobin	[26, 29]
	CCCAGGAGCTTGAAGTTCTCAGG
Hbb-b1	GCAGGCTGCTGGTTCTCTACC	165	59,9	Adult hemoglobin	[26, 30]
	TGCCCTTGAGGCTGTCCAAGTGA
HoxB4	TCACGTGAGCACGGTAAACC	119	62	Marker of haematopoiesis (adult hematopoietic stem cells)	[31, 32]
	CTCCCACCTCTAGCGGGTGC
IL3	CAGGGAGCAGAACCACGATAA	136	61	Hematopoietic cytokine	[33]
	CCTACAGACCGGATGGAGGA
IL6	TCTATACCACTTCACAAGTCGGA	88	59	Hematopoietic cytokine	[33]
	GAATTGCCATTGCACAACTCTTT
M-CSF-R	GGTTGTAGAGCCGGGTGAAA	233	60,9	Receptor for monocyte/macrophage colony-stimulating factor; marker of monocyte/macrophage development	[34, 35]
	AAGAGTGGGCCGGATCTTTG
PU.1/Spi-1	GTTTCCTACATGCCCCGGAT	178	61,6	Downstream of Runx1	[36]
	TTTTCTTGCTGCCTGTCTCCC
Runx1/AML1	AGGCAGGACGAATCACACTG	177	57,9	Marker of endothelial-hematopoietic transition	[37, 38]
	CTCGTGCTGGCATCTCTCAT
Sca1	GGACACTTCTCACACTACAAAG	161	56	Marker of HSC	[17]
	TAACACAGACTCCATCAGGGTAG
SCL/Tal1	TATGCCCCAGGATGACGGAG	73	60,1	Hematopoietic marker; upstream of Runx1	[24, 39]
	GCGTCCTGTCCCTCTAGTTG
p38α	GATTCTGGATTTTGGGCTGGCTCG	369	55	Verification of lineage
	ATCTTCTCCAGTAGGTCGACAGCC