NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells

Figure 1

Gene expression of HES1, HEY1, and HEY2 1 and 24 h after a single loading session in left-loaded and right-nonloaded osteocyte-enriched tibiae of 10-week-old female C57BL/6 mice. Fold changes in gene expression of loaded limbs () normalized to control limbs are shown (a) 1 h and (b) 24 h after loading. qPCR data were obtained from technical duplicates. Results are shown as ; fold change was calculated with the method and normalized to basal expression (nonloaded right limb, dashed line). GAPDH served as the housekeeping gene. Two-tailed paired -test was used for statistical analysis ( and ).

(a)

(b)