NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells

Figure 2

NOTCH target gene expression after cyclic stretching of BMSCs derived from five donors (donor #01-donor #05). (a) Relative mRNA expression of the NOTCH target genes HES1, HEY1, HEY2, and HEYL; the NOTCH1 and NOTCH2 receptor genes; and the NOTCH ligand gene JAG1. (b) Relative mRNA expression of the early osteogenic transcription factor RUNX2. Samples in (a) and (b) were collected 4 h after cyclic stretching. (c) Relative mRNA expression of the mechanoresponsive genes PTGS2 and FOS was analyzed 15 min and 4 h after cyclic stretching. qPCR data were obtained from technical triplicates (a, b) or from technical triplicates derived from three independent qPCR (c). Results are shown as ; fold change was calculated with the method and normalized to basal activity (nonstretched, dashed line). RPS27A served as the housekeeping gene.

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