NOTCH Signaling Is Activated through Mechanical Strain in Human Bone Marrow-Derived Mesenchymal Stromal Cells

Table 1

Primer names, sequences, product lengths, annealing temperatures, and GenBank accession numbers are shown.

Gene name	Primer	Sequence 5-3	Product length	Annealing temp (°C)	GenBank accession number
Primers designed by the Universal ProbeLibrary System Assay
HES1	HES1 FOR_h	GGAAATGACAGTGAAGCACCT	78	63.7	NM_005524.2
	HES1 REV_h	CAGCACACTTGGGTCTGTG
HEY1	HEY1 FOR_h	GGCAGGAGGGAAAGGTTACT	79	53.6	NM_001040708.1
	HEY1 REV_h	CTCAGATAACGCGCAACTTC
HEY2	HEY2 FOR_h	CCCGCCCTTGTCAGTATC	60	54.5	NM_012259.2
	HEY2 REV_h	TTGTTTGTTCCACTGCTGGT
HEYL	HEYL FOR_h	GTCCCCACTGCCTTTGAG	88	54.9	NM_014571.3
	HEYL REV_h	ACCGTCATCTGCAAGACCTC
JAG1	JAG1 FOR_h	GGCAACACCTTCAACCTCA	103	55.5	NM_000214.3
	JAG1 REV_h	GCCTCCACAAGCAACGTATAG
NOTCH1	NOTCH1 FOR_h	CGGGGCTAACAAAGATATGC	68	54.5	NM_017617.3
	NOTCH1 REV_h	CACCTTGGCGGTCTCGTA
NOTCH2	NOTCH2 FOR_h	TGGTGGCAGAACTGATCAAC	78	63.4	NM_024408.3
	NOTCH2 REV_h	CTGCCCAGTGAAGAGCAGAT
RPS27A	RPS27A FOR_h	TCGTGGTGGTGCTAAGAAAA	141	60	NM_001135592
	RPS27A REV_h	TCTCGACGAAGGCGACT
RUNX2	RUNX2 FOR_h	CTTCACAAATCCTCCCCAAG	147	58	NM_001024630.3
	RUNX2 REV_h	ATGCGCCCTAAATCACTGAG
HES1	HES1 FOR_m	CGGTCTACACCAGCAACAGT	88	66	NM_008235.2
	HES1 REV_m	CACATGGAGTCCGAAGTGAG
HEY1	HEY1 FOR_m	CCTTTGAGAAGCAGGGATCT	142	65	NM_010423.2
	HEY1 REV_m	CCCAAACTCCGATAGTCCAT
Primer sequences obtained from published studies
HEY2 [31]	HEY2 FOR_m	TCCACCTCTCTTCTGTCTCTTTCG	190	69	NM_013904.1
	HEY2 REV_m	GACTGGAGGCTGCGGATACC
GAPDH [32]	GAPDH_FOR_m	TGCGATGGGTGTGAACCACGAGAA	130	68.9	NM_008084.3
	GAPDH_REV_m	GAGCCCTTCCACAATGCCAAAGTT
Primers obtained from QIAGEN
PTGS2	Hs_PTGS2_1_SG	QIAGEN sequence		59	NM_000963
FOS	Hs_FOS_1_SG	QIAGEN sequence		57	NM_005252.3