The Analysis of In Vivo Aging in Human Bone Marrow Mesenchymal Stromal Cells Using Colony-Forming Unit-Fibroblast Assay and the CD45^lowCD271⁺ Phenotype

Figure 1

Age-related decline in number and proliferative capacity of BM MSCs. (a) Correlation between CFU-Fs/ml of BM aspirate with donor age across complete age range (left, donors, 19-89 years old) and between three age groups (right); black lines indicate the line of the best fit (left) and median values for each group (right). (b) Representative colony dish showing the presence of large and small colonies (left) and comparison of colony areas in all colonies in 7 young donors (19-37 years old, 321 colonies) and 7 old donors (61-76 years old, 266 colonies) (right). Each dot represents a single colony; black lines represent median values. (c) Frequency distribution of colony areas for colonies from the same 7 young (black curve) and 7 old donors (grey curve) (left) and comparison of integrated densities (IDs) of smaller colonies (left) and larger colonies (right) in the young and old donor groups. , , , and .

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