The Analysis of In Vivo Aging in Human Bone Marrow Mesenchymal Stromal Cells Using Colony-Forming Unit-Fibroblast Assay and the CD45^lowCD271⁺ Phenotype

Figure 2

MSC colony formation in media containing human serum. (a) Total number of colonies from 6 young (19-37 years old, left) and 4 old donors (65-79 years old, right), grown in StemMACS (SM) or media containing young donor serum (YS) or old donor serum (OS). (b) Cell counts from cells grown in each of the serum conditions in the same 6 young (left) and 4 old (right) donor groups. Bars indicate median values and whiskers represent interquartile ranges. (c) Frequency distribution of colony areas for all colonies from the same 6 young (left) and 4 old (right) donors grown in SM, YS, or OS conditions. (d) Comparison of IDs of all colonies from the same 6 young donors grown in SM (345 colonies), YS (286 colonies), and OS (301 colonies) conditions. (e) Comparison of IDs of all colonies from the same 4 old donors grown in SM (241 colonies), YS (175 colonies), and OS (170 colonies) conditions. Data in (d) and (e) is presented on log₂ scale. Black lines represent median values. and .

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