The Analysis of In Vivo Aging in Human Bone Marrow Mesenchymal Stromal Cells Using Colony-Forming Unit-Fibroblast Assay and the CD45^lowCD271⁺ Phenotype

Figure 4

Surface marker expression in CD45^lowCD271⁺ cells. (a) Gated populations and marker histograms from representative young (19 years old) donor showing MSCs (top panel) and HLCs (bottom panel) with median fluorescence intensity (MFI) indicated on the right top corner. (b) Gated populations and marker histograms from representative old (67 years old) donor showing MSCs (top panel) and HLCs (bottom panel) with MFI indicated on the right top corner. (c) Comparison of expression of selected surface markers in 8 young (19-38 years old, black) and 8 old (61-89 years old, grey) donor MSCs and HLCs with lines indicating median values with interquartile ranges for each group. CD271: CD45^lowCD271⁺ MSCs; HLC: CD45^highCD271^- hematopoietic lineage cells.

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