Atypical Mesenchymal Stromal Cell Responses to Topographic Modifications of Titanium Biomaterials Indicate Cytoskeletal- and Genetic Plasticity-Based Heterogeneity of Cells

Figure 1

(a) Assessment of cell adhesion at 1, 3, and 24 h post contact demonstrated adherence at near-maximal numbers to each of the test surfaces by 1 h. Cell numbers increased slightly on tissue culture plastic but remained unchanged on the Ti surfaces. There was a significantly lesser number of cells on the rough compared to polished Ti surfaces. Shown are , , . , , and for tissue culture plastic compared to Ti surface. , , and , P compared to SLA or modSLA Ti surfaces. , , and , SLA compared to modSLA Ti surfaces. (b) Assessment of MSC adhesion within the first hour of cell-substrate contact. The Calcein-AM cytoplasmic fluorescence labelling indicated a higher number of cells adherent to the polished Ti compared with TCP and both modified rough Ti surfaces. Shown are , , . , , and for polished Ti compared to any surface. (c) Back-scattered scanning electron micrographs of Calcein-labelled samples from one MSC donor. Images of samples from the attachment assay confirmed the presence of a higher number of MSCs adherent to P compared to the modified rough Ti surfaces. .

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