Research Article
Directional Topography Influences Adipose Mesenchymal Stromal Cell Plasticity: Prospects for Tissue Engineering and Fibrosis
Figure 3
Cell alignment (24 h of culture). (a) Overview of the Phalloidin staining (red) for F-actin on the two different wrinkle gradients (R1-R5 and R6-R11), as well as on the flat PDMS control. (b) Cell alignment quantification as the mean cell angle relative to the directional topography. The dotted line represents the 10° cutoff defining the limit for cellular alignment. Black represents the flat PDMS control, red represents the nanotopography gradient, blue represents the microtopography gradient, and green represents R11; vs. PDMS flat control. Values represent of 3 independent experiments.
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