Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells

Figure 4

The increase in intracellular ROS is dependent on bacterial incubation duration and bacterial strain. (a) After ASC staining with the ROS probe H₂DCFDA, cells were cultured with Ec, Fn, or Sg for 15, 30, 60, or 120 minutes and fluorescence was measured by flow cytometry (). (b) Before and during infection, the culture medium was supplemented with antioxidant (NAC, MnTBAP) or cytochalasin D, and the cells were incubated with or without Sg for 60 minutes and ROS production was measured using the H₂DCFDA probe (). The mean fluorescence values were given (arbitrary units). (c) Bacterial wall permeability of Sg and Fn induced by ASCs with or without antioxidants (NAC, MnTBAP) normalized according to the respective medium without ASCs and with or without antioxidants ().

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