Construction and Evaluation of a Subcutaneous Splenic Injection Port for Serial Intraportal Vein Cell Delivery in Murine Disease Models

Figure 1

Construction of the subcutaneous splenic injection port. (a) After hair removal from the surgical area, a subcutaneous pocket was created with a straight hemostat. (b) The spleen was translocated into the subcutaneous pocket. (c) The muscle layer was closed deep to the spleen. (d) Macro view of the translocated spleen. (e) The traced illustration indicates the avascular area of the splenic vascular pedicle. (f) Macro view of the closed muscle layer. (g) The traced illustration depicts the closure of the muscle layer supporting the spleen in the developed subcutaneous pocket. (h) One week following surgery. (i) hAECs were resuspended in 50% trypan blue/PBS solution and transdermally injected into the spleen. (j) One hour after the cell injection, no leakage was observed in the pocket.

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