Construction and Evaluation of a Subcutaneous Splenic Injection Port for Serial Intraportal Vein Cell Delivery in Murine Disease Models

Figure 3

GFP-labeled immortalized hAEC injection. (a) The low magnification fluorescent image (×10) of a recipient liver one hour after cell injection. (b) The high magnification fluorescent image revealed GFP-positive cells in the liver parenchyma (red arrow). DAPI (blue) and Alexa Fluor 594 Phalloidin (red) were used as counterstains to visualize the nuclear and liver structures, respectively. (c) Antihuman mitochondrion immunohistochemistry demonstrates cell distribution and microemboli in the mouse portal vein. (d) Antihuman mitochondrion immunohistochemistry demonstrates human cells in the mouse liver parenchyma.

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