Serum-Free Manufacturing of Mesenchymal Stem Cell Tissue Rings Using Human-Induced Pluripotent Stem Cells

Figure 4

Morphological changes of hiPSC-MSC tissue rings. (a) The morphology of hiPSC-MSC tissue rings before and after extraction indicated that ring shape turned into circular shape during continuous culturing. The tissue rings produced with low cell number (0.3M cells in total) showed no significant difference in (b) inner diameter and (c) ring diameter compared to standard cell number (0.6M cells in total). (d) The mini tissue rings and (e) asymmetric tissue rings were also fabricated with different designs of PDMS devices. Scale bar: 1 mm.

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