Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations

Figure 2

Characterization of SM-MPC, IFM-MSC, BM-MMSC, and AD-MMSC cultures expanded in vitro. (a) Representative histograms demonstrate the results of a comparative FACS surface marker analysis of SM-MPC, IFM-MSC, BM-MMSC, and AD-MMSC. Green histograms indicate the stained samples, and grey ones indicate the negative controls. The red ring indicates the CD56^bright subpopulations in SM-MPC and BM-MMSC samples (–5 in each group). (b) Visualization of FACS analysis in SM-MPC samples: CD56+/CD140a-, CD56+/CD140b-, and CD56+/CD146+ subpopulations are indicated in a rectangle gate; quadrant gates specify the negative/positive populations. Unstained cells were used as a negative control for each sample. (c) Immunocytological phenotyping of skeletal muscle precursor cells at early steps of expansion in vitro. Cells were stained for the expression of MYF5 (red; ~100% of positive), MYOG (red; 25± 4% of positive), vimentin (VIM, green; ~100 of positive), or CD146 (green; 20± 4.5% of positive). The insert demonstrates the enlarged region with a CD146+ cell. Nuclei were labelled with DAPI (blue). Scale bars represent 50 μm.

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