Perspectives for Clinical Translation of Adipose Stromal/Stem Cells

Table 2

Guidelines for immunophenotypic characterisation of adipose tissue-derived cells, modified from Bourin et al.’s study [14].

Feature	Assay	Cells of SVF	ASCs
Immuno-phenotype	Flow cytometry	Primary stable positive markers for stromal cells: CD13, CD29, CD44, CD73, CD90 (>40%), CD34 (<20%); Primary negative markers for stromal cells: CD31 (<20%), CD45 (<50%)	Primary stable positive markers: CD13, CD29, CD44, CD73, CD90, CD105 (>80% in ASC)
			Primary unstable positive marker: CD34 (present at variable levels)
			Primary negative markers: CD31, CD45, CD235a (<2%)
			Secondary other positive markers: CD10, CD26, CD36, CD49d, CD49e
			Secondary other low or negative markers: CD3, CD11b, CD49f, CD106, PODXL