Metformin Delays Satellite Cell Activation and Maintains Quiescence

Figure 6

Metformin delays skeletal muscle regeneration in vivo. (a) Experimental design of the in vivo administration of metformin to cardiotoxin- (CTX-) injured C57BL/6 mice. (b) Representative images of H&E staining on TA muscle sections of control and metformin-treated mice at 4 and 7 days of post-cardiotoxin (CTX) injury (DPI). (c) Quantitation of the centrally located myonuclei was performed on TA muscle sections upon H&E staining of control and metformin samples. The percentage of centronucleated myofibers is reported as the total number of centronucleated myofibers to the total number of myofibers in the damaged muscle area. Statistical significance was evaluated by the ANOVA test () (n control = 3 mice, n metformin = 3 mice). (d) Cross-sectional area (CSA) distribution of myofibers in the control and metformin-treated mice 4 days after CTX injury (n control = 3 mice, (). (e) Cross-sectional area (CSA) distribution of myofibers in the control and metformin-treated mice 7 days after CTX injury (n metformin = 3 mice, ).

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