Research Article
Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation
Figure 1
MSCs at P3 can spontaneously form spheroids in medium containing KSR. (a) Schematic diagram shows the experimental procedure; (b) hMSCs cultured in MiPS and MiPS without KSR/GLn/NEAA/βME/bFGF; (c, d) hMSC spheroids were generated and stained with Calcein-AM/PI in MiPS/KSR at days 1, 3, and 6 on tissue culture dishes. Statistical analysis of the mean diameter and cell viabilities of hMSC spheroids; sizes were measured from captured images of spheroids (-20); values are (); % of live cells (Calcein-AM/PI) was determined using flow cytometry; values are (). Scale bars: 100 μm.
(a) |
(b) |
(c) |
(d) |