Research Article
Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation
Figure 2
MSCs at higher passage retain the ability to form spheroids in medium containing KSR. (a) MSCs at P5 and P8 generated spheroids in MiPS at days 1, 3, and 6 on tissue culture dishes. Statistical analysis of the MSC spheroid mean diameter cultured in MiPS; (b) MSCs at P5 and P8 generated spheroids in L-KSR at days 1, 3, and 6. Statistical analysis of the MSC spheroid mean diameter cultured in KSR; sizes were measured from captured images of spheroids (-20); values are (). Not significant (NS); and . Scale bars:100 μm.
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