Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation

<div>Spheroid-recovered hMSCs retain mesenchymal stem cell features. (a) Cells in spheroids at day 6 at passage 8 in MiPS/L-KSR migrated out and adhered to tissue culture dishes when reseeded in L-FBS medium. Cells cultured in FBS as a control; (b) FACS analysis of MSC-positive markers CD73, CD90, and CD105 of spheroid-recovered hMSCs in MiPS/L-KSR. Cells cultured in L-FBS as a control. Scale bars: 100 <i>μ</i>m.</div>

Stem Cells International

fig5

Figure 5

Figure 5: Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation 