Endometriotic Peritoneal Fluid Promotes Myofibroblast Differentiation of Endometrial Mesenchymal Stem Cells

Figure 2

Isolation and identification of endometrial MSCs. (a-c) The normal, eutopic, and ectopic endometrial MSCs were identified for the expression of positive MSC markers CD44, CD73, CD90, CD105, CD146, and HLA-ABC and negative MSC markers CD14, CD31, CD34, CD45, CD117, and HLA-DR. Black lines, cells stained with a matched isotype control; red lines, cells stained with the antibodies indicated. EN-MSCs represent endometrial MSCs. (d) The normal, eutopic, and ectopic endometrial MSCs were identified for multipotency. The osteogenic differentiation was detected with alkaline phosphatase and alizarin red (×200), and the adipogenic differentiation was detected with Oil Red O (×400).

(a)

(b)

(c)

(d)