Endometriotic Peritoneal Fluid Promotes Myofibroblast Differentiation of Endometrial Mesenchymal Stem Cells

Figure 3

Endometriotic PF promotes myofibroblast differentiation of endometrial MSCs. (a) Endometrial MSCs from patients without endometriosis were treated with control PF or endometriotic PF, and the expression of collagen I, α-SMA, fibronectin, and CTGF was analyzed by Western blot. GAPDH was used as loading control. Con represents the cells treated without PF; ConPF and EMsPF represent the control PF and endometriotic PF, respectively. (b) The expression levels of proteins were quantified by densitometry and normalized to the expression of GAPDH. versus the cells treated without PF. versus the cells treated without PF. versus the cells treated without PF. versus the cells treated with ConPF at the same concentration. versus the cells treated with ConPF at the same concentration. versus the cells treated with ConPF at the same concentration. (c) Endometrial MSCs from patients without endometriosis were treated with control or endometriotic PF, and the expression of collagen I, α-SMA, fibronectin, and CTGF was examined by immunofluorescence analysis. The nuclei were stained with DAPI (×600). (d) Endometrial MSCs from patients without endometriosis were treated with control or endometriotic PF, and the expression of p-Smad2, p-Smad3, and Smad2/3 was analyzed by Western blot. GAPDH was used as loading control.

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