Human Liver-Derived Stem Cells Improve Fibrosis and Inflammation Associated with Nonalcoholic Steatohepatitis

Figure 2

Characterization of HLSC-master cell bank. (a, c) Representative confocal micrographs showing the expression of several hepatic, mesenchymal, and embryonic stem cell markers in HLSCs (original magnification at ×400). Three experiments were performed with similar results of HLSCs at passage 6 of the culture. (b) Representative FACS analyses of HLSCs showing the expression of albumin, CD29, CD73, and CD105 (red histogram). Yellow histograms represent isotypic control. Three experiments were performed with similar results of HLSCs at passage 6 of the culture. (d) Telomere length of HLSCs was measured using the Absolute Human Telomere Length Quantification qPCR Assay Kit. A single copy reference (SCR) primer set recognizes and amplifies a 100 bp long region on human chromosome 17 and serves as reference for data normalization. The reference genomic DNA sample with known telomere length (Ctrl) serves as a reference for calculating the telomere length of MCB.

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