Human Liver-Derived Stem Cells Improve Fibrosis and Inflammation Associated with Nonalcoholic Steatohepatitis

Figure 6

Effects of different amounts of HLSCs on liver function and morphology in MCDD-fed mice. (a) Histological quantification of fibrosis in MCDD-fed mice injected with different amounts of HLSCs (0.5, 1.5, or and , 9, and 8, respectively) or with vehicle alone at week 2, by multiphase image analyses of 10 fields per section (original magnification 400x). Data represents . ANOVA with the Newman-Keuls multicomparison test was performed: MCDD-fed mice injected with different doses of HLSCs versus MCDD-fed mice injected with vehicle alone (NASH). (b) Histological quantification of the surface area occupied by steatosis vacuoles in MCDD-fed mice injected with different amounts of HLSCs (0.5, 1.5, or and , 9, and 8, respectively) or with vehicle alone at week 2, by multiphase image analyses of 10 fields per section (original magnification 200x). Data represents . Albumin (c), alanine aminotransferase (ALT) (d), aspartate aminotransferase (AST) (e), and blood urea nitrogen (BUN) (f) were measured in the serum of control SCID mice (CTRL), in mice fed with MCDD for 4 weeks and injected with vehicle alone (NASH), and in MCDD-fed mice treated with intravenous injection of different amounts of HLSCs (0.5, 1.5, or and , 9, and 8, respectively) at week 2 and sacrificed at week 4. Data are expressed as . ANOVA with the Newman-Keuls multicomparison test was performed; HLSC-treated mice versus NASH mice treated with vehicle alone.

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