Stem Cells International / 2019 / Article / Fig 1

Research Article

Melatonin Rescued Reactive Oxygen Species-Impaired Osteogenesis of Human Bone Marrow Mesenchymal Stem Cells in the Presence of Tumor Necrosis Factor-Alpha

Figure 1

Melatonin decreased TNF-α-induced ROS generation during osteogenesis of BMSCs. BMSCs were cultured and underwent osteogenesis in osteogenesis medium containing vehicle (0.05 mol/L ethanol), TNF-α (10 ng/mL), or both TNF-α (10 ng/mL) and melatonin (100 μmol/L). (a) The adherent cells in a 6-well plate were incubated with DMEM containing CM-H2DCFDA (10 μmol/L) for 30 min at 37°C in darkness. Fluorescence was observed by microscopy in a representative sample on days 3, 7, and 14. Green fluorescence indicates ROS-positive cells. Scale bars: 200 μm. (b, c) The cells were resuspended in DMEM containing CM-H2DCFDA (10 μmol/L) and incubated for 30 min at 37°C in darkness. Measurement of ROS was analyzed with a flow cytometer. The graphs in (b) represent typical results of fluorescence intensity; the results in (c) are representative of three independent experiments. versus the vehicle group, versus the TNF-α group.
(a)
(b)
(c)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.