Research Article
Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration
Table 1
Protocol for stages 1-3 of the differentiation regime for hiPSCs.
| Growth factors | WNT3A (ng/ml) | Activin-A (ng/ml) | FGF2 (ng/ml) | NT-4 (ng/ml) | Follistatin (ng/ml) | BMP4 (ng/ml) | TGF-β1 (ng/ml) | GDF-5 (ng/ml) | BMP2 (ng/ml) | Stage | Day |
| 1 | 1 | 25 | 50 | | | | | | | | 1 | 2 | 25 | 25 | 20 | | | | | | | 1 | 3 | 25 | 10 | 20 | | | 40 | | | | 1 | 4 | | | 20 | 2 | 100 | 40 | | | | 1 | 5 | | | 20 | 2 | 100 | 40 | | | | 1 | 6 | | | 20 | 2 | 100 | 40 | | | | 1 | 7 | | | 20 | 2 | 100 | 40 | | | | 1 | 8 | | | 20 | 2 | 100 | 40 | | | | 2 | 9~30 | | | 20 | | | | 10 | 100 | 20 | 3 | 31 | Cultures terminated |
|
|