Intradiscal Injection of Induced Pluripotent Stem Cell-Derived Nucleus Pulposus-Like Cell-Seeded Polymeric Microspheres Promotes Rat Disc Regeneration

Table 1

Protocol for stages 1-3 of the differentiation regime for hiPSCs.

Growth factors		WNT3A (ng/ml)	Activin-A (ng/ml)	FGF2 (ng/ml)	NT-4 (ng/ml)	Follistatin (ng/ml)	BMP4 (ng/ml)	TGF-β1 (ng/ml)	GDF-5 (ng/ml)	BMP2 (ng/ml)
Stage	Day
1	1	25	50
1	2	25	25	20
1	3	25	10	20			40
1	4			20	2	100	40
1	5			20	2	100	40
1	6			20	2	100	40
1	7			20	2	100	40
1	8			20	2	100	40
2	9~30			20				10	100	20
3	31	Cultures terminated