Mesenchymal Stem Cells Derived and Cultured from Glioblastoma Multiforme Increase Tregs, Downregulate Th17, and Induce the Tolerogenic Phenotype of Monocyte-Derived Cells

Figure 6

GB-MSCs induce tolerogenic phenotype monocyte-derived cells. Representative dot plot analysis of monocyte-derived cell detection in the pool of PBMCs (a). Comparison of the percentage of CD14-positive monocyte-derived cells in the composition of control PBMCs, PBMCs cultured with the supernatant from GB-MSCs (), and PBMCs cocultured with GB-MSCs () (b). Comparison of the percentage of monocyte-derived cells expressing HLA-DR in monocyte-derived cells in the pool of control PBMCs and PBMCs cultured with supernatants of GB-MSCs () and PBMCs cocultured with GB-MSCs () (c). Comparison of the HLA-DR mean fluorescent intensity (MFI) in monocyte-derived cells in the pool of control PBMCs and PBMCs cultured with supernatants of GB-MSCs () and PBMCs cocultured with GB-MSCs (d). Comparison of the percentage of CD80- and CD86-expressing monocyte-derived cells in the composition of control PBMCs and PBMCs cultured with the supernatant from GB-MSCs ( and , respectively) and PBMCs cocultured with GB-MSCs ( and , respectively) (e).

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