Metastable Reprogramming State of Single Transcription Factor-Derived Induced Hepatocyte-Like Cells

Figure 3

Generation of dox-inducible iHeps. (a) Schematic diagram representing the procedure of d-iHep generation by the dox-inducible gene delivery. (b) The number of iHep colonies expressing albumin was counted after 15 days of transduction. Error bars indicate the standard deviation of triplicate values. and . (c) RT-PCR analysis describing the expression pattern of hepatocyte- and fibroblast-specific markers in the d-iHep lines. (d) Expression patterns of hepatocyte-specific markers in the d-iHep lines in the presence or absence of dox were compared by qPCR. Expression levels were normalized to d-iHeps cultured with dox. Error bars indicate the standard deviation of triplicate values. and . (e) The expression pattern of hepatocyte markers in 1a d-iHeps in the presence or absence of dox and small molecules. Error bars indicate the standard deviation of triplicate values. and . (f) In vitro functional analysis of 1a d-iHeps by PAS staining and ICG uptake in the presence or absence of both dox and small molecules. Scale bars: 100 μm. Error bars indicate the standard deviation of triplicate values. . (g) Percentage of PAS- or ICG-positive cells was measured. Error bars indicate the standard deviation of triplicate values. .

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